Submitted abstract:
Emerging
infectious diseases have been implicated as contributing factors to recent amphibian
population declines. One such disease reaching pandemic status is ranavirus, caused by a
group of viruses documented in fish, reptile, and amphibian species on five
continents. Mortality rates exceed 90% at the larval stage of many aquatic-breeding
amphibian species, including the common frog (Rana temporaria) in the
UK, tiger salamander (Ambystoma tigrinum) in Western US, and wood frog (Lithobates
sylvaticus) in Northeastern US.
As
part of a long-term wetland restoration ecological study beginning in 2010, The
Upper Susquehanna Coalition in collaboration with SUNY College of Environmental
Science and Forestry created a network of 71 hydrologically isolated pools
incorporating four pre-existing pools at Svend O. Heiberg Memorial Forest in
Tully, New York. One target species of the restoration, the wood frog, experienced
local die-offs of tadpoles in three pond sites in 2011, and preliminary testing
using polymerase chain reaction assay verified the presence of ranavirus in all
three sites. Two die-offs of wood frog and green frog tadpoles have since been
observed in other sites, with ranavirus confirmation pending.
To
identify environmental and organismal factors influencing susceptibility to ranaviral
infection at these sites, we are collecting ongoing data on tadpole
populations, environmental conditions, water quality, and ranavirus prevalence
both during and between epidemics. We will use general linear models to
determine which biotic and/or abiotic variables are most influential in
ranavirus outbreaks, to develop a predictive model that can be applied to other
landscapes. This will be especially applicable to future wetland restoration
endeavors, as the Heiberg system incorporates both natural and constructed
ponds and offers a unique opportunity to comparatively analyze disease
outbreaks in each.
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